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U ltrastructure. Three samples of the preparation of demineralized bone matrix described were prepared for examination by electron microscopy.
Before City Wildlife opened its doors in 2013, injured animals were taken to Second Chance Wildlife Center in Gaithersburg, Md. Now, City Wildlife treats everything from orphaned opossums to injured squirrels to snakes — a corn snake was brought in as the eagle was being prepared for examination — in its center on Oglethorpe Street NW.
The proximal tibia was dissected out and prepared for examination.
After 3 passages, the isolated cells were prepared for examination and analysis by flow cytometry.
Direct microscopic examination is a cheap methodology, the microscope slides can rapidly be prepared for examination, and there is no need for sophisticated laboratory equipment.
The tissues were then washed with 1xTBST and incubated for 30 minutes at room temperature with biotinylated secondary antibody solution from the Dako REAL EnVision Detection System (Dako, Denmark), washed with distilled water, counterstained with hematoxylin (SIGMA-ALDRICH, Inc., USA), dehydrated and clarified by a conventional method, and prepared for examination under a light microscope.
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Tumor samples were then taken and paraffin-embeded sections were prepared for immunohistopathological examination.
Formalin-fixed paraffin-embedded sections were prepared for pathological examination.
Tissue samples were paraffin-embedded and prepared for neuropathological examination.
At that point, tumor samples were taken, and paraffin-embedded sections were prepared for immunohistopathological examination.
Two skin biopsies of 1 3 mg were taken from the buttocks and prepared for microscopic examination, as described previously [ 15].
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