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A 21 nt hybridization sequence, 5′-GGAUGGCGACGGCAGCGAGGC-3′, was prepared for attachment at the 5′-end of P2−P3; the corresponding 18-mer DNA was derivatized with biotin at its 3′-end and had no Cy5 label.
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The P2-enriched beads were isolated from the upper glycerol layer, modified with a 3' amino group for surface attachment, and prepared for deposit on the SOLiD slide.
A period of oncology teaching appeared to improve preparedness: 44% of those who had done an oncology attachment felt prepared for looking after patients with cancer, compared with 34% of those who had not (χ=17.9; df=1; P<0.001).
DNA fragments containing the attachment sites attB and attP were prepared for use as substrates in EMSA [28], [42].
Bachrach and Zautra (1985), Tierney et al. (2001), Mishra et al. (2010), and Anton and Lawrence (2016) found that households with stronger attachment to a place or community were more likely to be prepared for disasters.
As a result, a biocompatible scaffold with good cell attachment, facility in formation in desired shapes and simplicity in production were prepared for bone tissue engineering.
In this case I found myself thinking: Maybe the attachment to stuff comes in part from a notion that we should be prepared for anything.
Bachrach and Zautra (1985), Tierney et al. (2001), Mishra et al. (2010), and Anton and Lawrence (2016) found that the stronger the place attachment or community ties of households, the more likely they were to be prepared for a disaster.
Australians' attachment to rural legends is exploited by local politicians to deliver their communities' wish lists — from counterproductive subsidies for farmers who haven't prepared for drought to the construction of an inland railway line of dubious value.
Be prepared for frustration.
Be prepared for honesty!
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com