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To compare the anticancer property of RGE, we prepared extracts of additional seven vegetables and 14 fruits in the way similar to that of RGE preparation.
The researchers prepared extracts from several types of hazelnut trees, and after purifying and analyzing the samples, Hoffman noticed the familiar chemical signature of paclitaxel.
We prepared extracts from single eggs and from single embryos in the earliest stages of development.
The Karginov et al. study employed similar growth conditions, and also prepared extracts 48 hours after transfections.
To follow-up on this observation, we prepared extracts from the glioblastoma cell lines M059K and M059J, which do or do not express DNA-PKcs, respectively, and immunoprecipitated with specific anti-DNA-PKcs antibodies.
Prepared extracts were re-dissolved in 60 µl of hexane and transferred into GCMS vials (Supelco).
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The yield of the prepared extract (% w/w), in terms of the starting material, was 16.3.
Then, cultures were treated for 24, 48, and 72 h with the previously prepared extracted media.
To prepare extracts, adherent cells were trypsinized, centrifuged, and washed in PBS.
We generally prepare extracts from the yeast "noodles" in batches of six.
A Mini Lyophilizer (LTE Scientific Ltd ,UK) and rotary flash evaporator were used for preparing extracts.
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