Sentence examples for prepared competent from inspiring English sources

Chemically prepared competent cells are more widely used and work well in most cases for penetration of the plasmids.

The recombinant pHSG415 plasmids (pHSG415 containing locked or unlocked alleles) were first transformed into chemically prepared competent E. coli DHα cells, and then purified and transformed via electroporation (Bio-Rad) into LT2 strains.

PCR products were purified from agarose gels using AxyPrep DNA gel extraction kits (Axygen) and an A-tailing nucleotide was added with Taq DNA polymerase (Promega) prior to cloning into pGEM T-easy vector (Promega) and transformation of chemically prepared competent E. coli DH5α cells.

The nursing faculty shortage challenges us to prepare competent students.

They also require a step of preparing competent cells which is a time consuming process [6, 7].

Briefly, strain grown in LB to an optical density of 0.3 0.4 at 600 nm was used to prepare competent cells.

Saccharomyces cerevisiae FY834 (MATα; his∆200; ura3-52; leu2∆1; lys2∆202) was refreshed on YPD agar medium (per liter: 20 g glucose, 20 g peptone, and 10 g yeast extract) at 28 °C for 48 h and then used for preparing competent cells as described by Frozen-EZ Yeast Transformation II Kit (T2001, Zymo research, USA).

The correct plasmid was transformed into strain SW106 to prepare competent cells after induction of the λ recombination genes.

To prepare competent cells, L. pneumophila grown on BCYE agar plates were resuspended in 200 ml of sterile water to an OD600 between 0,5 1.

Current resources and approaches are inadequate to prepare competent service providers.

Issues exist of funding, coordinating and managing CBE to prepare competent providers.