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One mL of the tested preparation could then be filtrated by adding it to the prepared column and centrifugating them for 1 minute at 700 g.
The prepared column was first characterized in terms of morphology, pore properties, capacity and retention mechanisms.
Wallis ordered the prepared column to be scrapped and replaced by one about an "evil conniving girlfriend who had got herself deliberately pregnant in order to crash this [the New York job]".
The chromatographic performances of the prepared column were investigated by reversed phase chromatography using alkylbenzenes and positional isomers, hydrophilic interaction chromatography using nucleosides, nucleic bases and flavonoids, and ion exchange chromatography using acidic and basic analytes.
Then dried methanol extract (4 mg) of M. fraxinea Sm. was first dissolved in methanol and carefully applied by pipette at the top of prepared column.
The prepared column had a better binding capacity towards glycoproteins compared with non-glycoproteins (Yang, Lin, He, Chen, and Zhang, 2011).
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These prepared columns had a threefold higher capacity compared to monoliths prepared in a multistep process involving Schiff-base DNA attachment.
The heights of prepared columns were 500, 600 and 900 mm, and the corresponding shear span-to-depth ratios were 1.42, 1.75 and 2.75, respectively.
After centrifugation at 15,500×g for 30 min, the clarified supernatant was applied to Protino®Ni prepared columns (Macherey-Nagel, Dueren, Germany) and the polyhistidine-tagged protein was eluted with 1×elution buffer (50 mM NaH2PO4, 300 mM NaCl, 250 mM imidazole, pH 8.0).
Brain homogenates were then added to the prepared columns (1.0 mL from rat and 0.7 mL from wild-type mouse).
To do so, we prepared column-depleted perforin-competent SCILs preparations deficient in CD8+ cells, CD4+ cells, or NK1.1+ cells.
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CEO of Professional Science Editing for Scientists @ prosciediting.com