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In galvanostatic charge/discharge cycles the prepared cell showed good electrochemical performance.
In addition, the prepared cell exhibited a long cycle-life of more than 2000 cycles at 25 °C.
We followed the protocol described by Shin et al.65 Briefly, ColI (BD bioscience) was buffered to a final concentration of 2 mg/mL, 4 mg/mL, or 6 mg/mL with 10x DPBS containing calcium and magnesium (Thermo Fisher), cell culture-grade water (Lonza), and a previously prepared cell solution.
Finally, the prepared cell suspension was loaded into the cell chamber.
The freshly prepared cell culture was dropped on a strictly cleaned glass plate immediately before the measurement.
Experiments were carried out with 20 mL of the prepared cell free extract put in 150 mL conical flask.
The prepared cell suspension was then centrifuged to discard the fixative, resuspended in 2 mL of PBS solution, and filtered using a 200-mesh cell screen.
Afterward, 200 μl of the prepared cell solutions were added to polystyrene TC-treated 96-well microplates (Corning ® Life Sciences Corning, NY, USA, #3603).
A total of 0.2 mL of the prepared cell suspension (4 × 105 HepG2 cells) was injected into the right armpit of each nude mouse and tumor growth observed every other day.
Total RNA was extracted from above prepared cell samples and infarcted heart tissue respectively.
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FTIR was performed on the prepared cell-wall biomass for all miscanthus samples (25 lines × 3 time points × 2 tissues × 3 plant replicates).
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