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At the beginning of her first-grade year, a new IEP for Rowley was prepared, as required by the EHA.
Human influenza vaccine reference strains are prepared as required when an antigenically new strain is recommended by WHO for inclusion in the vaccine.
The cells were then harvested and extracts prepared as required.
Solutions were prepared as required, sterile-filtered and stored at 2 4°C for maximally one week.
Stock solutions (30 mg/ml in ethanol with 250 μg/ml BHT) of 7-KC, 7βOHC and 7αOHC (an optical isomer of 7βOHC) were freshly prepared as required.
Sodium dithionite solutions (10% w/v) were prepared as required and used within 3 h.
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Plasma samples were removed from −80°C storage, carefully thawed on ice, and prepared (diluted as required) according to the manufacturer's instructions.
PdNi precursor solution was prepared as follows: required amounts of the metallic salts of Pd and Ni to obtain 100 mg of bimetallic were dissolved in deionized water.
It was prepared fresh as required and kept at 37°C with an osmolarity in the range of 290 310 mOsm/kg.
A 5 m m stock solution of pyrocatechol violet (PV) indicator was prepared freshly as required using this buffer solution.
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CEO of Professional Science Editing for Scientists @ prosciediting.com