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Fresh lymph nodes were obtained from the surgically resected specimens and prepared as outlined in methods.
Guanidine thiocyanate solution (1.0 ml) and silica bead (Sigma) suspension (10 µl), prepared as outlined by Boom et al. 1990 [28], were added to the sample.
Compound C425 was prepared as outlined below.
Alginate-coated glass surfaces were prepared as outlined in Supplementary Fig. 2a.
Principal component analyses were performed to examine any variation and clustering between PAS of tumorgrafts and GSE44076 using the prcomp function of the 'stats' package in R. PASs were prepared as outlined above.
NPSiT were prepared as outlined by Asli et al. [19].
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Call, write, or visit as outlined above.
RNA isolated from dormant teliospores, filamentous diploid cultures and haploid cells grown in CM, MC, or MN media, was used to prepared cDNA as outlined for the RT-qPCR analysis.
Samples were prepared and reacted as outlined in the Experimental Section and two droplets were generated for each sample.
A prepared breakfast meal, as outlined below, was administered consistent with the first diet assignment, and hourly blood samples were collected for 5 h to study the postprandial breakfast response.
Equal amounts of total RNA from wild-type and transgenic mammary glands (distinct from that prepared for the microarray hybridizations) were pooled as outlined in Additional file 1 and then treated with DNaseI.
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