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Working solutions were prepared as directed with MT1-MMP substrate.
Broth was prepared as directed (30 g/L) and without sterilization.
Controls and standard curves were prepared as directed by the supplier.
A 30-μL sample was added to 1.5 mL of Coomassie Reagent (Coomassie Brilliant Blue G-250 dye) prepared as directed and vortexed.
Mounts were then subjected to graded ethanol to water washes, and stained with carmine solution (Sigma, C1022 prepared as directed, with Aluminum Potassium Sulfate, Sigma, A7167) overnight with shaking.
Polydimethylsiloxane (Dow Corning, MIDLAND, MI, USA) was prepared as directed by the manufacturer and poured to a depth of 5 mm over the photoresist master, the chamber was degassed and left overnight to cure.
Similar(52)
In a statement Mr. Sorkin said: "I am glad to be surrounded by such a supportive group of people and am prepared to proceed as directed by my attorney and in the best interest of my family.
Mr. Cicero did as directed.
Analogue 8xvi (Entry 18) was prepared as a direct comparison with 8ii (Entry 4).
The constructs with the common haplotypes were: pGEM-ACG (-20 = A, -44 = C, -52 = G), pGEM-GCA and pGEM-GGG; an additional construct, pGEM-GCG, was also prepared as a direct control for pGEM-GGG, even though this haplotype has not been observed in the general population [ 31].
Crust: 1 recipe Olive Oil Double Crust prepared and rolled as directed (see below).
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