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Post sterile additions for a batch medium were prepared as concentrated stocks, filter sterilized, and injected into the bioreactor through a septum on the head plate.
Inhibitors were prepared as concentrated stock solutions in DMSO, stored at -20°C, and diluted in culture medium at the time of use.
Solid stocks were purchased from the indicated suppliers and prepared as concentrated stock solutions in the appropriate solvent: gemcitabine (Apin Chemicals Inc), 20 mM in H2O; cisplatin (Selleckchem), 3.33 mM in 1% NaCl in H2O; oxaliplatin (Tocris), 5 mM in H2O; carboplatin (Tocris), 25 mM in H2O; PF-477736 (Selleckchem), 20 mM in DMSO and AZD7762 (Axon Medchem), 20 mM in DMSO.
Similar(57)
"Twice as concentrated as Colgate".
Be prepared as soon as possible.
Complex I was prepared as described previously, concentrated to approximately 10 mg/mL (determined by the Pierce bicinchoninic acid assay), snap-frozen in liquid nitrogen, and stored at −80 °C.
As long as I concentrated.
Concentrated methanolic extract of B. serrata was prepared as described.
Concentrated supernatant protein samples were prepared as described above and samples were run on 10% gels.
Concentrated stocks of recombinant BV expressing scFv/p17 molecules, BV-scFvG2/p17 and BV-scFvE2/p17, respectively, were prepared as follows [ 41].
The ethanol extract was prepared as follows: The dried and pulverized medicinal herbs were mixed together, and each batch was poached twice for a total of 3 h, then filtrated, concentrated, and soaked with ethanol for 12 h.
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