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"Anyplace in the country that says they are 100percentt prepared, absolutely they are not".
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Being prepared is absolutely right.
Just be prepared for absolutely nothing to change, because if there's one thing that teenagers love more than themselves, it's ignoring rules. .
RNA samples were prepared using Absolutely RNA® Microprep or Nanoprep spin columns (Stratagene, USA) and cDNA synthesized from 500 ng RNA using SuperScript III reverse transcriptase (Invitrogen, Life Technologies, UK). Real-time PCR reactions were run in triplicate and CT values obtained using a MX3000P real-time PCR machine (Stratagene, USA).
We must build on the solid foundations we have laid down over the past eight months and prepare an absolutely irresistible licence application for the future".
He was absolutely prepared.
"Egypt was absolutely prepared for the revolution".
I'd speak to people who'd say they were pretty sure it happened on such and such a date, but they weren't prepared to say absolutely.
Mayor Rudolph W. Giuliani, wearing a commemorative Mets cap in the Champagne-soaked home clubhouse, said, "We're prepared for an absolutely incredible Series".
I absolutely prepared for that meeting and RBS sponsored me for three years, giving me time to set up a team and a sustainable model to deliver my idea.
Bundy confirmed that fire observation towers at the refuge were now occupied by marksmen, for "safety", and said he was "absolutely" prepared to use force if he thought the situation warranted it.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com