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We prepared a source of [C]-R5P using a modified process as described in Boss et al. (1984), for the subsequent use in Mt-PrsA assays.
Starting with [C]-U d-glucose and replicating the first three enzymic reactions of the pentose phosphate pathway in vitro using commercially available enzymes (Roche and Sigma), we prepared a source of purified [C]-U-α d-R5P as a substrate for subsequent enzyme assays.
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And this week, as I prepare to lose a source of income that allows me the financial freedom to write professionally, Jefferson's words haunt my brain.
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The results were compared to a standard curve prepared using K2HPO4 as a source of inorganic phosphate at a concentration ranging from 0.0448 to 2.8706 μM.
One drawback of this approach is the patchwork image it impresses upon the modern student, as if Ibn Kammuna prepared a quiltwork of philosophical sources rather than taking a stand on the issues.
The public should be made aware that hamburgers, even when prepared at home, can be a source of infection.
As part of that decision, budget officials prepared a menu of possible funding sources for the restoration, including anti-pollution grants, state water bond revenue, soil cleanup funds and even a local tax increase.
Last year, NYU's Furman Center prepared a report on New York City, source of much of the rhetoric around gentrification, that charted changes in rental value from 1990-2010.
Enzyme solution was prepared with rabbit liver microsomes as a source of glucose-6-phosphatase.
Assays were conducted using membranes prepared from M. smegmatis which provided a source of DPPR synthase (Huang et al. 2005), which was necessary to investigate incorporation of [C]-R5P into the lipid-linked organic extractable material.
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