Sentence examples for prepare the sequencing from inspiring English sources

These bacteria most likely grew in the samples during the prolonged storage before extraction of the nucleic acids used to prepare the sequencing libraries.

The Illumina mRNA-Seq Sample Prep Kit (Illumina, San Diego, CA USA) was used to prepare the sequencing library with 3 μg of nuclear RNA.

The ABI PRISM BigDye cycle-sequencing kit (Perkin Elmer, Foster City, CA), and specific primers, or the primers for the M13 forward and reverse priming sites of the pCR2.1 vector, were used to prepare the sequencing reactions.

The shorter than expected reads could have been due to a combination of factors in preparing the samples for sequencing such as the T7-based antisense RNA amplification method, the conversion of antisense RNA to cDNA, or during the shearing process of the cDNA to prepare the sequencing library.

"During the Mars night we prepare the sequences the rover has to do," he explains.

The problem is that even the locations of each fragment within gp120 vary between samples, requiring significant manual curation to prepare the sequences for comparison.

To prepare the sequence template (nucleotides 75-638 of 5'NTR of HAV), PCR products were treated with ExoSAP-ITR (Affymetrix, Inc., Santa Clara, CA), and then sequenced using a BigDye(R) Terminator v3.1 Cycle Sequencing Kit (Life Technologies, Tokyo, Japan).

The Pregap4 modules were used to prepare the sequence data for assembly (quality analysis).

The trial biostatistician will prepare the sequence of treatments randomly permuted in blocks of unequal size.

The EST cleaning pipeline described by Baudet and Dias [ 67] was then used to pre-process the ESTs and prepare the sequences for assembly.

GH prepared the sequencing samples, participated in the bioinformatic analyses, conducted the genotyping, and evaluated the genotyping data.