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Clinical WES therefore requires thorough genetic counseling to prepare subjects for unexpected findings and to explain results.
Premotor cortex activation during the mental rotation of objects could therefore prepare subjects for the possibility of acting on them [ 6].
The investigator should have prior knowledge of the available methods for assessing IR, their limitations and how to prepare subjects prior to investigation.
In a study to prepare subjects to run a half-marathon, 9 out of 32 subjects withdrew on being unable to keep up with the training programme (Westerterp et al. 1992).
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In the study preparing subjects to run a half-marathon, subjects with the highest BMI dropped out (Fig. 4).
Blood samples were collected from all 21 animals, following which blood smears were prepared, subjected to quick Romanowsky staining and examined under the optical microscope.
Titanium complexes based on the (R,R -N,N′-bis 2-hydroxy-3-functionalised-benzylidene -1,2-diaminocyclohexane R,R -N,N′-bis 2-hydroxy-3-functionalised-benzylidene -1,2-diaminocyclohexane R,R -N,N′-bis 2-hydroxy-3-functionalised-benzylidene -1,2-diaminocyclohexane R,R -N,N′-bis 2-hydroxy-3-functionalised-benzylidene -1,2-diaminocyclohexane R,R -N,N′-bis 2-hydroxy-3-functionalised-benzylidene -1,2-diaminocyclohexane R,R -N,N′-bis 2-hydroxy-3-functionalised-benzylidene -1,2-diaminocyclohexane R,R -N,N′-bis 2-hydroxy-3-functionalised-benzylidene -1,2-diaminocyclohexane R,R -N,N′-bis 2-hydroxy-3-functionalised-benzylidene -1,2-diaminocyclohexane R,R -N,N′-bis 2-hydroxy-3-functionalised-benzylidene -1,2-diaminocyclohexane
Cell lysates were prepared, subjected to the MT-2 pull-down assay, and analyzed by immunoblotting as described previously [16], using approximate 450 µg of protein lysate per assay.
Tumor lysates were prepared, subjected to SDS-PAGE, transferred to nitrocellulose and analyzed by immunoblot analysis [ 9].
Nuclear extracts were prepared, subjected to immunoprecipitation with an anti-HA antibody, and Western blotted as indicated.
Cryosections of adult testicular tissue samples were prepared, subjected to direct histochemistry and subsequently micro-dissected as previously described (Sonne et al, 2009b; Jorgensen et al, 2011).
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