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Some amount of engineered NPs was re-suspended in ultrapure water for further characterization, while other NPs were lyophilized to prepare stocks (trealose was added as cryoprotector).
To prepare stocks of these viruses for the neutralization studies, 1 ml aliquot of culture supernatant containing the virus isolated from patients' PBMCs was used to infect the PBMCs of 3-day phytohemaglutinin-stimulated HIV-negative pooled donors [19], [35].
Escherichia coli BE (su −, Epstein et al. 1963) was the host bacterium used to prepare stocks of all of these phages.
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These standards were used to prepare stock standards solutions.
Chloroauric acid was dissolved in ultrapure water to prepare stock solution for further use.
All conventional antifungal agents were dissolved in 96%% ethanol to prepare stock solutions (10.24 mg ml−1).
Meanwhile, these four pure ingredients were dissolved by DMSO to prepare stock solutions and then diluted as needed.
Accurately weighed 50 mg of TAP was dissolved in triple distilled water to prepare stock I (1,000 μg/ml) in 50-ml volumetric flask.
Accurately weighed 50 mg of OLO was dissolved in 50% aqueous methanol to prepare stock I (1,000 μg/mL) in a 50-mL volumetric flask.
All inhibitors were dissolved in DMSO to prepare stock solutions.
To prepare stock cultures, a preculture in TB medium was made.
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