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First, we could prepare passage 0 synovial MSCs, expanded with autologous human serum in 14 days, for transplantation.
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The cells were furthermore amplified during 5 passages for preparing passage 33 (P33) samples for omics analysis.
In the first step the cells were amplified during 4 passages for preparing passage 27 (P27) freezing stocks.
In addition, the ability to prepare enough passage 0 cells in 14 days could reduce costs compared with the need to passage cells multiple times for longer periods.
hESC were mechanically dissociated and passaged onto ECM coated plates with CM prepared from passage 11 (CMp11) or passage 18 HFFs (CMp18).
The task force hopes to have legislation prepared for passage by the end of February.
The White House forecast was prepared before passage of the huge housing assistance package that Mr. Bush has promised to sign.
For the synthesis of Dawson acid, at first potassium salt was synthesized and then Wells Dawson acid, [H6P2W18O62], was prepared by passage of potassium salt solution in water through a column of Dowex resin (H+ form).
According to the number of clusters N obtained after the integration of the clustering operator, the SA creates N cluster agents (CAs) preparing the passage to the next step of the global algorithm.
Morard de Galles had spent most of 16 December preparing for passage through the Raz de Sein, situating temporary lightships in the channel to warn of hazards and giving instructions on the use of signal rockets during the passage.
After digestion lungs were prepared by passage through 100 µm cell strainer into supplemented DMEM.
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