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To prepare for scanning, induction of anesthesia was accomplished with 2% isoflurane and 1 l/min oxygen.
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Tissues were prepared for scanning electron microscopy (SEM) and stereology.
Samples were prepared for scanning transmission electron microscopy using a modified focused ion beam liftout procedure.
Raw and metal-adsorbed BSDs were dried and prepared for scanning electron microscopic studies.
Finally, the samples were prepared for scanning electron microscopy (SEM) and transmission electron microscopy (TEM) according to the previously published method (Taute et al. 2015).
Microcolonies were prepared for scanning electron microscopy (SEM) as follows.
Samples were then prepared for scanning electron microscopy (SEM) as described in the Materials and Methods.
Embryos were then fixed and prepared for scanning electron microscopy (EM) as previously described [55].
Up to 30 specimens each of the four gregarine species were prepared for scanning electron microscopy (SEM).
In parallel experiments, samples were prepared for scanning electron microscopy (SEM) studies as follows: biofilms on the coverslips were fixed for 24 h in a solution containing 2.5% glutardialdehyde.
Myxospores from Striped Marsh frog's gallbladder were prepared for scanning electron microscopy (SEM); placed on poly-L-lysine coated coverslips, fixed in 1% OsO4 in 0.2 M sodium cacodylate buffer (pH 7.0), rinsed in distilled water, dehydrated with a graded acetone series, critical point dried, and coated with gold.
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