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The rotation of the substrate about two axes during the deposition procedure could prepare condition for production of three-dimensional structures with anisotropic columns in 1 100 nm scale [10].
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The job of the transitional government will be to lead the country in the 10 weeks until elections and prepare conditions for a fair vote, something that was markedly lacking in the federal elections.
To prepare conditioned media, 3 million cells were plated in a T-150 flask.
To prepare conditioned media and cell lysates, BAE cells were grown in 6-well plates.
To prepare conditioned medium, subconfluent cultures were incubated in RPMI-1640 medium with 0.1% FBS for 24 h.
To prepare conditioned growth medium, one part of the supernatant was diluted in two parts of unconditioned medium.
To mimic the uterine local environment, uterine stromal cells were isolated, cultured and used to prepare conditioned medium (CM).
Malignant epithelial cells derived from NPC xenografts – LMP1-positive (C15) or negative (C17) – were used to prepare conditioned culture medium.
To prepare conditioned medium, PRF membranes were soaked in 5 mL fresh DMEM medium without fetal bovine serum in 6-well cell culture plates.
To prepare conditioned serum, 35 ml of whole blood was acquired through venapunction and aspirated in six polypropylene syringes (5 ml) containing glass beads (Orthogen, Düsseldorf, Germany).
To prepare conditioned media, HMFs were plated into duplicate wells of a six-well plate and exposed to each agent or corresponding vehicle controls 24 hours later.
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