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Following library preparation, Ion OneTouch system was used to prepare a template and sequencing was performed using an Ion 316 chip on an Ion PGM sequencer and results were analysed using Ion Ampliseq CHPv2 regions and hotspots, hg19 as reference in single sample analysis.
A clone was grown in the presence of kanamycin, purified using a Qiagen maxi prep kit, and the resultant plasmid linearized with SpeI to prepare a template that made a 768 base antisense riboprobe that was used without hydrolysis.
Prepare a template in advance.
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For grant proposals that do require a description of RCR training, FAS Research Administration Services has prepared a template with suggested language regarding RCR training.
We prepared a template file for A. thaliana microarray ATH1.
In March 2009, the Laboratory for the Development of Substitute Materials (LDSM) marooned itself for ten days on a mesa in Arizona.2 We had, for the previous several months, been preparing a template for a structured response to that environment upon arrival.
Nested PCR is a recognized highly sensitive technology for HPV DNA detection and for preparing a template for DNA sequencing.
The implant placement plans were then established, and the data were sent to a preparation center to prepare a surgical template.
Fifty individuals of C. sinicus from the same population (the Yellow Sea) were pooled to prepare a DNA template for mitogenome sequencing.
We prepared a damaged template DNA produced with an alkylating agent (N-methyl-N-nitroso urea; MNU) to measure incorrect base-incorporation during DNA replication in microgravity.
The TPM library prepares a command template for each TPM command in the runtime stack.
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