For the λ-phosphatase treatment of Cat1-GFP, cells were broken with glass beads in buffer A without NP-40 (Nakashima et al., 2010) by vortexing twice for 5 minutes at 4°C and were centrifuged at 14,000 g for 5 minutes at 4°C to prepare a membrane-rich fraction.
But if you have the time to prepare it, a rich stock made with one of the ingredients in the gumbo can add a more robust flavor.
Just as importantly, we believe that our mission is to help students prepare for a rich, meaningful and engaged life that goes well beyond job titles and salary levels.
To prepare a cup of juice rich in potassium, place some carrots, celery, spinach, parsley, and green algae in a blender.
Here we prepare a series of surface Pd rich CuxPdy/C catalysts with different Pd to Cu ratios which may be applied in methanol and ethanol oxidation in alkaline media.
For the Belle II spectrometer we are preparing a proximity focusing RICH with aerogel as the radiator.
Prepare for a rich, dynamic immersion.
To prepare cells under a rich condition, they were resuspended in 100 ml of YPD at an OD600 of 0.1 and grown at 30°C for 6 hrs.
A dainty portion of salt-baked trout, also finished in the oven, is unusually delicate, prepared not in a rich French style but with preserved lemons, black olives, and plenty of chervil.
During this time, the practices of the providers were observed and field notes were prepared to provide a rich description of the setting as well as to note specific details including the timing, nature of the interaction and the outcome.
