Sentence examples for prepare a pool of from inspiring English sources
Are increased computational costs associated with "on the fly" ligand flexibility in docking justified or is it enough to prepare a pool of low energy conformers and reduce a rather complex docking procedure to "simple" accommodating of prepared conformers (without further modifying their geometry) into the protein binding site (with side-chains being either rigid or flexible)?
The pSM2 library version 1.3 comprising 15,148 constructs targetting 9,392 human cancer associated genes was amplified and each 96 well plate used to prepare a pool of plasmid DNA; each of the 100 pools contained between 150 to 200 different shRNAmir constructs with each gene being represented by 1 to 3 shRNAmirs.
To test this, we prepared a pool of normalized DNA from the same five individuals previously analyzed separately (pool size of 10 alleles).
In order to facilitate comparison of data between the three separate length fractionations, an internal standard was prepared with a pool of in vitro-transcribed Methanococcus jannaschii RNAs.
A serial dilution standard curve was prepared from a pool of all the samples and real-time PCR was carried out.
For normalization between the length fractions, an internal standard was prepared with a pool of in vitro-transcribed Bacillus subtilis RNAs [18].
Total RNA was prepared from a pool of 20 wild type and 20 EP-704/EP-704 3rd instar larvae using the TRIzol reagent RNA protocol following GibcoBRL instructions.
For sample A, protein extracts were prepared from a pool of 40 male antennae (20 specimens) and from a pool of 80 female antennae (40 specimens), being female antennae smaller and less plumose than males.
Each soluble intestinal contents sample was prepared from a pool of 20 midguts from blood unfed A. aegypti females with at least 7 days old, or a pool of 6 anterior midguts from each triatomine species obtained from starving fourth instar nymphs with 10 to 20 days after moult.
Total RNA was prepared from a pool of T2 transgenic plants (n > 50 for each line).
For each time point, RNA samples were prepared from a pool of ~30 plants per replicate.
