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Final purification of hAPP-TM was achieved by preparative reversed-phase high performance liquid chromatography (HPLC).
It was then purified by preparative reversed-phase high-performance liquid chromatography (RP-HPLC).
We previously showed that insoluble 68Ga-species can be removed from the labeling reaction of 68Ga-labeled DOTA-exendin-3, a tracer targeting the glucagon-like peptide-1 receptor (GLP-1R), using (preparative) reversed-phase high-performance liquid chromatography (RP-HPLC) [3].
Crude peptides were purified by preparative reversed-phase HPLC to >95% purity, and were lyophilized.
Fractions 3 to 6, rich in stilbenoids, were further purified by semi preparative reversed-phase HPLC to give compounds 1 (70 mg), 2 (35 mg) 3 (19 mg), 4 (8 mg) and 5 (8 mg).
Peptides were purified by preparative reversed-phase HPLC using dual wavelength detection (228 nm: amide; 360 nm: Sox).
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The crude solution was purified on a Luna C18 semi-preparative reversed-phase HPLC column (Phenomenex, 5 μm, 250 × 10 mm) with a mobile phase (acetonitrile/water = 20 80) delivered at 5 mL/min.
The [11C]-methyl triflate reacts with the precursor in presence of a base at room temperature for 2 minutes gives the carbon-11 radiolabeled [11C]BA-1 wasch was purified by using semi-preparative reversed-phase high pressure liquid chromatography (RP-HPLC).
Peptides were purified using a C18 semi-preparative reversed-phase column (Merck, Darmstadt, Germany) by HPLC, to a >95% purity, and their identity was confirmed by Mass Spectrometry.
Rh-ET-1 was purified using semi-preparative reversed-phase HPLC using a Vydac C-18 column (250×10 mm, 10 µm).
Two purest gamma-oryzanols (γ-oryzanols) (>98 wt%) were initially obtained by preparative reverse-phase high-performance liquid chromatography.
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