Sentence examples for preparative reverse-phase from inspiring English sources

Two purest gamma-oryzanols (γ-oryzanols) (>98 wt%) were initially obtained by preparative reverse-phase high-performance liquid chromatography.

C3-1 wasubsequentlyly purified by preparative reverse-phase HPLC eluted with 11%% MeCN/H2O (+0.2 % FA) to give compounds 3 (9 mg, tR = 18.5 min), 4 (13 mg, tR = 20.5 min), C3-3 wasubsequentlyly purified by preparative reverse-phase HPLC eluted with 14%% MeCN/H2O (+0.2 % FA) to give 7 (12 mg, tR = 27.5 min).

C4-1 wasubsequentlyly purified by preparative reverse-phase HPLC eluted with 12%% MeCN/H2O (+0.2 % FA) to give compound 5 (8 mg, tR = 25.5 min) and 6 (16 mg, tR = 26.5 min).

B3 was chromatographed on a Sephadex LH-20 column (2 × 150 cm) eluted with MeOH system and then was purified by preparative reverse-phase HPLC eluted with 40%% MeOH/H2O (+0.2 % formic acid (FA)) to give compound 15 (7 mg, tR = 23 min) and compound 16 (8 mg, tR = 19 min).

E3 was purified by preparative reverse-phase HPLC eluted with 18%% MeCN/H2O (+0.2 % FA) to give compounds 14 (tR = 26.5 min) and 8 (tR = 30.5 min), and then compounds 14 and 8 were purified by a Sephadex LH-20 column (2 × 40 cm) eluted with MeOH system to give compounds 14 (8 mg) and 8 (6.5 mg), respectively.

B8 was chromatographed on a Sephadex LH-20 column (2 × 150 cm) eluted with MeOH system and then was purified by preparative reverse-phase HPLC eluted with 50%% MeOH/H2O (+0.2 % FA) to give compound 17 (6 mg, tR = 31 min).

The reaction was stirred and heated at 150°C for 5 min before the reaction mixture was diluted with mobile phase (500 μL) and purified by semi-preparative reverse-phase chromatography.

The crude reaction mixture was diluted with 200 μL water before injecting into a high-performance liquid chromatography [HPLC] semi-preparative reverse-phase μBondapak column (C18, 7.8 Ø × 300 mm, 10 μm, Waters Corporation) for purification.

Semi-preparative reverse-phase HPLC was conducted using an Agilent Eclipse XDB-C18 column (9.4 × 250 mm, 5 μm) coupled to an Agilent 1200 LC system, with a 3 mL min−1 flow rate and UV spectroscopic detection at 220 nm.

ACP and RGV performed semi-preparative reverse-phase HPLC for isolation of EVOO polyphenols and coordinated the chemical (ACP, RGV, AGF, ASC) and biological (JAM, AVM, COF) study groups.

Reaction mixtures containing 200  μ M CB1954, 1 m M NAD(P H and 1  μ M NfsA or NfsB in 10 m M Tris pH 7.0, 5% DMSO were incubated at room temperature for 5 min, before separation by semi-preparative reverse-phase HPLC as described earlier (Race et al, 2007).