Sentence examples for preparative purification from inspiring English sources
We detected the expression of 30 of these targets and describe the preparative purification of two arbitrarily selected proteins.
Countercurrent chromatography (CCC) is a separation technique in which two immiscible liquid phases are used for the preparative purification of synthetic and natural products.
In order to obtain large quantity of N16 for animal experiment and clinical trial, a fermentation and preparative purification method was established.
Monolithic media were compared with Q- and SP-Sepharose high performance chromatography for preparative purification and with Q- and SP-5PW chromatography for analysis of a pegylated form of myelopoietin (MPO), an engineered hematopoietic growth factor.
The results have indicated that this process made good use of the high feed load, low solvent costs, and high resolution characteristics of displacement chromatography and offered the chromatographic engineer a powerful tool for the preparative purification of therapeutic compounds.
However, these approaches suffer from two drawbacks; proteins with a translocated C-terminus cannot be tested and scale-up from analytical to preparative purification is often non-trivial and may require re-cloning.
Fig. 6 a Semi-preparative purification of the product [18F]EFB with HPLC method A (Table 1).
The initial [18F]F- activity was 37 ± 3 GBq (range, 32 to 42 GBq), and the average synthesis time was 43 ± 3 min, including the synthesis of [18F]F2, radiofluorination, and semi-preparative purification.
The synthesis and purification of [18F]F-Gln is more complicated than the other radiotracers described in this study, requiring more than the typical semi-preparative purification and Sep-Pak isolation/elution.
Purification methods encompassed preparative HPLC purification using a Varian Prostar system with two Prostar 210 pumps and a Prostar 325 UV/Vis detector, using a Phenomenex Luna C18 column (250 × 21.2 mm, 10 μm) using method 1: A-H2O with 0.1% trifluoroacetic acid (TFA) and as mobile phase B-CH3CN with 0.1% TFA.
Alternatively, preparative HPLC purification was performed on a Gilson PLC 2020 personal purification system using a Nucleodur VP 250/32 C18HTec 5 μm column from Macherey-Nagel at a flow rate of 32mL/minn.
