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Displacement chromatography is an efficient mode of preparative chromatography.
Pulse response experiments were performed on 105 preparative chromatography columns with volumes of 0.2 20 ml.
With respect to batchwise preparative chromatography, simulated moving bed (SMB) units exhibit a number of advantages.
Sample solubility in the mobile phase and enantioselectivity are key factors in chiral preparative chromatography.
The potential of applying nonlinear gradients in preparative chromatography is evaluated theoretically and experimentally.
Fibers are prominent among novel stationary phase supports for preparative chromatography.
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For preparative chromatographies, a third washing step with 60 mM imidazole was added and the protein was eluted by 5 - 10 ml of glycerol buffer containing 250 mM imidazole.
The product was purified by semi-preparative chromatography using method A (Table 1, Fig. 6a) and identified by co-elution with an authentic reference compound with method B (Table 1, Fig. 6c).
Optimization of the purification of biopharmaceuticals by preparative liquid chromatography including an examination of column preparation and bed properties is the focus of this manuscript.
The lower the required limit of quantitation of PAHs, the larger is the scale of preparative liquid chromatography in both steps of sample preparation needed.
Increasingly the potential of modulating the solvent strength during gradient operation is exploited in preparative liquid chromatography.
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