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Four other preparations were tested for 1 min.
At least 5 different TcSn preparations were tested.
All preparations were tested in cell cultures and found to be nontoxic at the concentrations used in the present study.
Sonication has been shown to increase HA binding inhibition activity [24], so HA preparations were tested plus or minus sonication.
RNA preparations were tested for the lack of Brucella genomic DNA contamination by PCR with primers specific for the IF-1 Brucella gene.
Recombinant HMGB1 preparations were tested routinely for LPS content by the chromogenic Limulus amebocyte lysate assay (Endochrome; Charles River), and endotoxin content was below detection limit (<500 pg endotoxin per microgram of rHMGB1).
Similar(37)
The reusability of the immobilized enzyme preparations was tested by performing five sequential activity assays using DMP as the substrate.
The activity of the purified IgG and F ab' 2 preparations was tested in hemagglutination inhibition (HI) and virus neutralization (VN) assays against PR8 virus in vitro.
The purity of the subunit preparations was tested by SDS-polyacrylamide gel electrophoresis and the absence of the nucleoprotein and matrix protein of the subunit preparations was tested by western blotting using monoclonal antibodies against the influenza A nucleoprotein and the influenza A matrix protein.
The cell specificity of our RNA preparations was tested by RT-qPCR.
The absence of DNA contamination in RNA preparations was tested by including RNA samples that had not been reverse transcribed.
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