Sentence examples for preparations were separated from inspiring English sources

Mitochondrial preparations were separated on standard 12% glycine SDS polyacrylamid gels.

LPS preparations were separated by SDS-PAGE using a 4% polyacrylamide stacking gel and a 15% polyacrylamide separating gel.

Briefly, intact borrelial cells were incubated with either proteinase K or trypsin and whole-cell protein preparations were separated by SDS-PAGE (13%) as described [26].

Amplicons generated from reactions with or without reverse transcriptase, to confirm absence of genomic DNA in RNA preparations, were separated in ethidium bromide-stained 0.8% agarose gels.

The purified LPS preparations were separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) using a 4% stacking layer and a 15% separation gel.

2G12 preparations were separated by SDS-PAGE under reducing conditions [55] and either stained with Coomassie blue for direct visualisation or electroblotted onto nitrocellulose.

In brief, 2.5 OD260 units of each ribosomal protein preparation were separated in the first dimension in a 4% (w/v) polyacrylamide gel containing 8M urea at pH 8.6.

The aliquots of DNA preparation were separated by electrophoresis on 1% agarose gels, stained with ethidium bromide, and photographed by a UV transilluminator.

Each preparation was separated into a soluble stromal and membrane fraction and then resolved by 1D SDS-PAGE, followed by in-gel trypsin digestion and identification by online nano-liquid chromatography tandem mass spectrometry (nanoLC-MS/MS) with an LTQ-Orbitrap, using data dependent acquisition (DDA) and dynamic exclusion.

An equal amount of protein (30 µg/sample) of each tissue extract preparation was separated on 14% SDS-PAGE, followed by immunoblot analysis with rabbit anti-human cathepsins S, L, and K polyclonal antibodies (1∶1000, Calbiochem, San Diego, CA) or rabbit anti-human cystatin C polyclonal antibodies (1∶1000; DAKO Corp., Carpinteria, CA), all of which cross-reacted with mouse gene products.

For immunoblot analysis, an equal amount of protein from each cell type preparation was separated by SDS PAGE, blotted, and detected with mAbs against mouse FcεR1α (1:1,000; eBioscience, Inc).