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PolyP preparations were resolved on urea-containing 15% polyacrylamide gels and visualized using DAPI negative staining as described.
Our Aβ preparations were resolved by 12% Bis-Tris gel electrophoresis and electrotransferred to polyvinylidene difluoride (PVDF) membranes, which were probed with a mouse monoclonal anti-β Amyloid antibody (clone BAM-10, diluted 1 2,000: Sigma Aldrich).
Approximately 30 μg of total protein preparations were resolved by SDS-polyacrylamide gel electrophoresis and subsequently electro-transferred overnight onto nitrocellulose membranes of 0.45 μm pore size (Schleicher and Schuell GmbH, Dassel, Germany).
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The enriched preparation was resolved by denaturing gel electrophoresis, and was found to contain two major proteins with apparent molecular masses of 75 and 60 kDa.
20% of the ConA preparations and 1% of lysates were resolved on SDS-PAGE gels for subsequent western blotting.
The same mitochondrial preparations used for activity measurements were resolved by SDS-PAGE according to [ 40], and western blotted as in [ 41].
Those issues were resolved.
None were resolved.
Also, binary mixtures of atrazine alachlor and atrazine bentazone in pesticide preparations have been resolved.
Mitochondrial preparation (70 µg/lane) was resolved in a 10% SDS-polyacrylamide gel and catalase was detected with the Athens' polyclonal antibody as described above.
The issue has cast a huge shadow over their preparations and had still to be resolved when they trained at the Estádio Nacional on Wednesday night.
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