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RNA extractions yielding insufficient quantities for TruSeq preparations were processed using the SMARTer Ultra Low RNA Kit (Clontech, Mountain View, CA) (Method ID: SMART_dT).
All preparations were processed in parallel and images acquired with identical settings using the 40× or 50× (zoomed 1 4×) objectives of a Leica TCS SP2 confocal microscope.
When the primary anti-cell marker antibodies were from rabbit, we used the two steps sequential process described previously [43]: tissue preparations were first treated for ET immunodetection and then, after a 20 min post-fixation in paraformaldehyde 4%, the preparations were processed for cell marker detection.
100 µm vibratome sections and crypts-villi preparations were processed for immunofluorescence in solution.
For size separation of total lipoproteins, preparations were processed within a week.
The metaphase preparations were processed using the Quantitative Image Processing System Applied Imagingg, Santa Clara, CA).
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The preparation was processed in a KCl molten salt at a temperature range of 650 950 °C using soluble low-molecular-weight phenolic resol as carbon source.
One RNA sample of each preparation was processed without RT-reaction to provide a negative control in subsequent PCR.
Plasmid preparation and sequencing were processed at the Iowa State University Office of Biotechnology DNA facility.
For plasma preparation, blood samples were processed within 30 minutes by centrifugation at 2,000 × g for 15 minutes at 4°C to obtain plasma.
RNA preparations
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