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Details on fly husbandry used for the RNA preparations were previously described (Sturgill et al. 2013).
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Methods for tagging and library preparation were previously described in [ 46] and followed manufacturer's instructions.
Compound stock preparation was previously described54.
The detailed protocol for liposome preparation is previously published.
The detailed method of OMCS preparation was previously reported [ 14– 17].
Details of the experimental preparation are previously described (Sato et al. 1996; Ozeki et al. 2004).
TNF-related apoptosis-inducing ligand preparation was previously described by Cha et al (1999).
Preparations that were previously air-dried were washed with demineralized water, dried again, and embedded in Vectashield.
All instrumental methods including sample preparations were as previously described except for TEM.
Tissue culture procedures and culture media preparation have been previously described [5].
The method of HLXL preparation has been previously reported [26], [27].
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