Six Natto preparations were obtained, generated by using different B. subtilis strains.
NET, DAT and SERT expressing membrane preparations were obtained from Perkin Elmer (MA, USA).
Following the manufacturer's protocols, total RNA preparations were obtained using Purelink a Trizol RNA isolation and purification kit (Invitrogen, USA).
Enzyme preparations were obtained as culture supernatants from 78 fungal isolates grown on wheat straw as carbon source.
Preparations were obtained squashing immature anthers in a drop of a 2% propionic acidhaematoxylin solution and using ferric acid as mordant (Núñez 1968).
Leukemic cells were treated with C60-RITC for 2 and 18 h, washed from excess of label and fixed preparations were obtained.
Cellophane tape preparations were obtained from the treated rats on day 0 pre-treatment and on days 2, 4 and 6 post-treatment.
By the classical spectrophotometric method based on absorbance at 260 nm of NHS released upon the reaction with ammonia, consistent molar equivalents of these two NHS esters of mPEG5k in their preparations were obtained, correspondingly (data not given).
For the latter, membrane preparations were obtained from capsule-plus-epithelial specimens, and from three cortical fractions, i.e. the anterior, equatorial, and posterior regions, as well as a fifth, nuclear fraction.
Plasma membrane preparations were obtained from roots of barley (Hordeum vulgare L. cv. Minorimugi) and 50 μM CdSO4 with or without 50 μM CuSO4 were added to the PM suspensions.
Crude membrane preparations were obtained as previously described [50].
