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Sample preparations were modified based on Lu et al. (2007).
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To determine the active range of the vibratory signal the neurophysiological preparation was modified to record multi-unit action potential activity of vibration receptors in the leg nerve of D. gigliotosi.
The sample preparation was modified according to literature [ 48, 49] and described as follows.
For cytosolic cytoskeletal fractionation, cytoskeletal preparation was modified from Zeidman et al. Briefly, cells were lysed for 20 min on ice with the lysis buffer mentioned in the western blot section.
Four different types of OMLS have been used for the preparation of nanocomposites, three were modified with functionalized ammonium salts while fourth one was a phosphonium salt modified OMLS.
The organoclays used in preparation of the nanocomposites were modified with ammonium surfactants of different molecular structures to study the effect of the surfactant on the intercalation and exfoliation of the polymer by X-ray diffraction (XRD) and dynamic linear viscoelastic analysis.
Sample preparation methods for MS were modified from Shevchenko et al. [ 43].
In their preparation, the inorganic titanate nanofibers were modified first by block copolymer and then PANI was coated by chemical oxidative polymerization of aniline monomer in the presence of modified titanate nanofibers.
The fabrication process involved the preparation of zinc acetate particles that were modified on TiO2 nanotube arrays.
Related to this point, the authors need to characterize and describe the labeling of their actin with atto488/565: how many sites on average were modified, what was the variability from preparation to preparation, what collection of sites were modified?
The three cruisers were modified to resemble destroyers, and where thoroughly overhauled in preparation for the attack.
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