Sentence examples for preparations were mixed from inspiring English sources

For Western blot analysis, the soluble and insoluble protein preparations were mixed separately in the same volume of loading buffer, separated on SDS/PAGE gels and then transferred onto nitrocellulose membrane.

The two preparations were mixed at 0°C, okadaic acid (Calbiochem) was added to 0.04 µM, and PMA (Calbiochem; kindly provided by Xiao-Hui Zhang) was added to 0.5 µM.

In brief, the immunoprecipitated samples of active ERK preparations were mixed with Mg2+/ATP cocktail, the Assay Diluition Buffer I (ADBI, n. cat. 20 108, Upstate) and the MAPK substrate cocktail II (n. cat. 20 166, Upstate); then, the reaction mixtures were incubated for 20 30 min in a shaking incubator kept at 30°C.

Equal amounts of the two preparations were mixed and used in hME reactions as described in Methods section "MALDI-TOF MS genotyping of adjacent SNPs".

Aliquots of extended semen or centrifuged sperm preparations were mixed with TNE buffer (0.15 M NaCL, 0.01 M Tris-HCL, 1 mM EDTA (ethylenediaminetetra-acetic acid), pH 7.4) to a final sperm concentration of approximately 2 × 106 cells/mL.

Frozen mycelia samples were disrupted with a single 5 mm stainless steel bead (Qiagen), whereas thawed conidia preparations were mixed with approx. 150 μL of 625 μm glass beads (Sigma).

For western blotting, aliquots of total protein preparations or membrane protein preparations was mixed well with an equal volume of 2× SDS loading buffer containing 100 mM DTT, maintained at room temperature for 30 min before loading onto 8% Precise Protein Gels (Pierce , Life Technologies Grand Island, NY, United States).

The falafel balls taste as though the mashed chickpeas in the preparation were mixed with bread; nonetheless, they are tasty, filling and a steal.

For combined RNAi (co-RNAi), approximately equal amounts of each bacterial preparation were mixed before spreading on the plates.

After coupling with HMGCR or SRP, 10 μl of each bead preparation were mixed and incubated with serum samples in the same conditions than described previously.

In a 20-μl assay volume 8 μl of the rPrP/antibody mixture, 2 μl compound (final concentration in primary screening: 10 μM), and 10 μl of the diluted PrPSc preparation were mixed.