Sentence examples for preparations were loaded from inspiring English sources

In order to compare the effectiveness of immobilized lipases in transesterification assays, all enzymatic preparations were loaded of 2U lipase, calculated from their hydrolytic activity when immobilized.

Aliquots of ~3 μg RNA per lane (L. donovani axenic amastigote total RNA from a single culture, or exosome RNA pooled from 4 separate exosome preparations) were loaded onto 8% denaturing polyacrylamide gels.

Sucrose gradients (10 50%) were generated using a Biocomp Gradient Master, and protein preparations were loaded and centrifuged at 35 000 rpm for 2 h 40 min. Polysome gradients were then loaded to the fractionator to obtain the translational profiles.

10 μg of protein from cell lysates or 5 μg of protein from crude mitochondrial preparations were loaded on 12% or 18% Tris-Glycine gels, proteins were transferred to PVDF membranes and then incubated with the indicated antibodies at 4°C overnight.

Briefly, equal quantities (8  μg) of the same preparation were loaded on two 1D-PAGE gels and submitted to electrophoresis.

The optimum conditions for the preparation were loading amount of 2 wt%, calcination at 700 °C in air followed by reduction at 400 °C in 5%H2.

After dialysis, the protein preparation was loaded onto anion exchange UNOsphere Q (Bio-Rad), and cation exchange UNOsphere S (Bio-Rad) columns (column volume, 15 mL).

The dialyzed crude preparation was loaded onto a preactivated DEAE-cellulose column (15 × 2 cm) equilibrated with 50 mM phosphate buffer (pH 7.8).

In the first step, the crude preparation was loaded onto a Q Sepharose FF column (anion exchanger XK 26/20, GE Healthcare) and the proteins were eluted with a linear gradient of 0-0.7 M NaCl in 10 mM sodium acetate buffer (pH 5.5) at a flow rate of 6 ml min-1.

Total RNA was quantified by spectrophotometric analysis (λ = 260 nm), and 5 µg of RNA of each preparation was loaded onto a 1% agarose gel and separated in 10 mM sodium phosphate buffer as described previously [20].

The preparation was loaded onto 1 mL HiTrap NHS-activated HP coupled with purified recombinant CT.