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Lipopeptide preparations were initially analyzed by HPLC to ensure purity of compounds used for subsequent experiments.
All preparations were initially perfused in normocapnic conditions (pCO2 = 40 mmHg) and maintained at a continuous positive airway pressure (CPAP) of 5 cmH2O.
All preparations were initially fractionated on a semiprep Vydac C18 column, and the collected main peaks were then separated on an analytical Luna C18 column.
Dilution series of total gluten protein preparations were initially analysed, allowing a concentration to be selected which was within the linear response region for all ω-gliadin bands.
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For example, we found that after talking without preparation participants were initially unable to sleep because they had recalled and relived the worst moments of their lives.
During the root canal preparation, the root canals were initially irrigated with 1 mL 60°C 3% NaOCl (Septodont, Saint-Maur, France) for 1 min after each instrumentation, and then with 5 mL sterile distilled water followed by 1 mL 17% EDTA (META, Chungbuk, ROK) as the final rinse after the whole instrumentation process.
During the root canal preparation, the root canals were initially irrigated with 1 mL 60°C 3% NaOCl for 1 min after each instrumentation, and then with 5 mL sterile distilled water followed by 1 mL 17% EDTA as the final rinse after the whole instrumentation process.
German losses were initially heavy due to Soviet defensive preparations.
As previously described [ 5], each heart-lung preparation was connected to a ventilator and the lungs were initially recruited with continuous positive airway pressure (CPAP) of 20 cmH20.
Approximately 200 seeds were initially sown on each of 120 replicate plates in preparation for sub-culturing.
This led to its participation in the preparations for the 1981 Operation Opera to destroy Iraq's Osirak nuclear reactor, which the Hammers were initially planned to conduct.
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