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The crude carbohydrate preparations were fractionated on the Sephadex G-50 column, and corresponding fractions screened by 1H-NMR and composition analysis (Figs. S1 and 2).
(b) Ultracentrifuged cell-free preparations were fractionated by gel filtration chromatography.
Preparations were fractionated using detergent-free methods as we have described previously [23].
The dsRNA preparations were fractionated on 1.0% agarose gel and stained with ethidium bromide.
Final preparations were fractionated by SDS-PAGE and analyzed by silver staining (top panel) and by Western blot (bottom panels).
Briefly, muscle myofilament preparations were fractionated by electrophoresis on a 12% 200 : 1 bis-acrylamide Laemmli gel with a 4% 29 : 1 bis-acrylamide stacking gel.
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For zymogram staining, the crude enzyme preparation was fractionated by native polyacrylamide gel electrophoresis (PAGE) using 10%% acrylamide as resolving gel and 4%% stacking gel (Laemmli 1970).
Gradients were fractionated with fraction collector Model 2128 (Biorad).
The polymers were fractionated by fractional precipitation.
For detection of short (~23nt) RNA species total RNA preparations from Drosophila cell cultures were fractionated by chromatography using sepharose CL-2B agarose (Sigma) in Micro Bio-Spin columns (Bio Rad), following the manufacturers instructions.
CSF peptides were fractionated into 30 fractions.
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