Sentence examples for preparations were first from inspiring English sources

To dehydrate, the preparations were first rinsed in 1×PBS and then incubated overnight in 24% sucrose in 1×PBS at 4°C.

Finally, in order to eliminate contamination by endotoxin, these Trx80 preparations were first passed through a polymyxin B column and then in a positively charged sterile filter.

When the primary anti-cell marker antibodies were from rabbit, we used the two steps sequential process described previously [43]: tissue preparations were first treated for ET immunodetection and then, after a 20 min post-fixation in paraformaldehyde 4%, the preparations were processed for cell marker detection.

The heart preparations were first characterized without ischemia.

Preparations were first fractionated by SDS-PAGE and subsequently transferred to nitrocellulose and immunoblotted.

Surface preparations were first captured using a × 40 magnification lens on the Zeiss microscope.

Preparations are first scanned at low magnification (× 10) to identify cells carrying both immunolabelled markers.

The best characterized of these are sighs (Ramirez and Viemari, 2005), which are generated within the medulla, persist in reduced preparations, are first seen during late embryonic stages, and are increased after SP application (Lieske et al., 2000; Gray et al., 2001; Ballanyi and Ruangkittisakul, 2009; Chapuis et al., 2014).

The buffer of the crude BG preparation was first changed to 50 mM sodium acetate (pH 5) containing 0.15 M NaCl using a Toyopearl HW-40 column.

To reduce pHi, H+ ions were injected by iontophoresis, while to increase pHi the preparation was first equilibrated with bicarbonate-buffered saline and then returned to normal snail Ringer's solution.

The different protein antigens (50 600 μg/ml) in phosphate-buffered saline (PBS) (or 0.1 mM NaHCO3, in case of amylase) were coated on the plates overnight at 4°C (in case of Rabies, the preparation was first incubated at 37°C for 2 hrs).