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Preparations were filtered to remove debris and were resuspended in RPMI.
Preparations were filtered to remove debris and were resuspended in RPMI with 10% FCS.
The strom and epi preparations were filtered with 40-μm Falcon cell strainers before labeling for MACS.
After enzymatic treatment, skeletal preparations were filtered through a 40-μm cell strainer and undigested bone material was discarded.
At these two time intervals, the preparations were filtered through a 0.8/0.45 μm filter and the target compounds were measured in the filtrates.
In the current study risk factors for aggregation were avoided; preparations were not heated or stored in conditions reported to contribute to protein aggregate formation and preparations were filtered prior to injection [ 33].
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Each media preparation was filtered, and dialyzed fetal bovine serum (dFBS) was added to a final volume of 10%, Glutamax was added to a final concentration of 1X, and Penicillin/Streptomycin was also added to a final concentration of 1X.
Following endotoxin removal, the final preparation was filtered through a sterile filter.
The liposome preparation was filtered with a membrane filter (0.22 μm pore size) and used.
After the preparation was filtered, the M. charantia L. extract was stored at 4°C.
The TcSC preparation was filtered (100 nm) by a standard Cardinal Health protocol to produce filtered Technetium-99m Sulfur Colloid (fTcSC).
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