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The rUbi and rMERS-NP preparations were extracted and analysed with 12% SDS-PAGE.
Insulin preparations were extracted from beef or pork pancreas until recombinant DNA technology made it possible to manufacture human insulin.
Aqueous preparations were extracted by stirring in a covered beaker for 1 h at room temperature.
Such membrane preparations were extracted with Triton-X-100 and passed over a DEAE cellulose column; the DEAE unbound fraction and the bound fraction eluted with 0·4M NaCl were used to immunize rabbits.
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These standard LPS preparations are extracted by a phenol-water mixture and contain other bacterial components, such as lipopeptides, and therefore stimulate TLR2 as well as TLR4.
Total RNA from cell or exosome preparations was extracted using the miRNeasy kit (Qiagen) and quantified with a NanoDrop1000 spectrophotometer (Witec AG).
The aqueous preparation were extracted in distilled water at 100 °C for 120 min.
Phospholipids (PLs) constituting the BAP preparation were extracted from hen egg yolk with ethanol and purified using acetone precipitation according to standard procedures [ 27].
Samples for RNA preparation were extracted from exponentially growing cultures at OD 0.1, and for RNA and DNA preparation from stationary phase at OD 0.6 for S. acidocaldarius and at OD 0.7 for S. solfataricus.
Tong Luo Jiu Nao injection (TLJN), a Chinese medicine preparation, was extracted from the Chinese herbs Panax notoginseng and Gardenia jasminoides.
The enzyme preparation was extracted by addition of 0.05 M acetate buffer and filtered using muslin cloth.
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