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NLC preparations were diluted with double-distilled water.
Before being injected into LC-MS/MS, the radiotracer preparations were diluted adequately to avoid possible saturation of the ionization and detection systems.
SPION stock solutions (3 mgFe/mL) and the supernatants of the SPION preparations were diluted to 1 16.6 and 1 1.6, respectively using sterile deionized H2O.
As negative controls, HZ preparations were diluted in serum-free DMEM.
All toxin preparations were diluted in 1X phosphate-buffered saline (PBS), filter-sterilized, frozen and thawed only once prior to use.
A. aegypti mitochondrial preparations were diluted to 100 µg/mL in potassium phosphate buffer 100 mM (pH 7.2) and 5% Triton X-100.
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One of the two highly pure HRV-A2 preparations was diluted in 50 mM NH4OAc (pH 8.4) and the other one in 10 mM NH4OAc (pH 9.5), yielding final virus concentrations of 3 and 16 nM, respectively.
The standard solutions that were previously prepared as a bulk preparation were diluted into murine DNA from untreated E- and P-selectin-deficient scid mice.
10 to 100 µl of the colanic acid preparation were diluted to 1 ml with distilled water, and mixed with 4.5 ml of H2SO4/H2O (6∶1 v/v).
The DNA preparation was diluted with ddH2O (3:100), and 1 μL of the diluted DNA was used for PCR analysis.
Briefly, the lentiviral preparation was diluted tenfold serially in PBS (from undiluted to a dilution of 10-5).
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