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In 32 sera (88.9%), antibodies reactive with both preparations were detected.
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Antibodies against TG antigen preparation were detected in 52 (14.5%, 95%CI11.0%0%–18.6%) of 357 specimens tested; 47 (90.4%) of these specimens that reacted to TG antigens were also positive for SFG antigens, and 5 (1.4%) of the 357 specimens were positive for TG antigens alone.
To investigate the change of the down-stream apoptostic protein after expressions of PrP mutants, the cellular level of pro-caspase-3 protein in each preparation was detected by Western blot.
We identified 34 proteins (or protein subunits) in DF preparations, while in WF preparations, interactions were detected with only 4 proteins.
Preparation errors were detected during the preparation by the double checked of the fabrication process and by self-reported by pharmacy technicians, or at the time of final pharmaceutical control.
As with the previous preparation, LMW species were detected by SE-HPLC whicomprisedsed approximately 34% of the digest mixture.
At a higher temperature (450°C), equal to the final calcination temperature during catalyst preparation, anionic vacancies were detected at the surface of TiO2 and are in equilibrium with gaseous oxygen.
As previously reported by others for different membrane preparations [ 26], both subunits were detected in CM and BLM with β1 in larger amounts in BLM.
In these RNA preparations, high levels of FMDV RNA were detected (Ct <20, see Table 2).
When virus particles were probed with antibodies for ppUL83 or ppUL32 similar amounts of proteins were detected in all preparations.
Robust bands were detected in those preparations that displayed the most robust cDNA smears and bands were routinely not observed with the media controls (data not shown).
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