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Post holes preparations were created according to manufacturer's instructions.
In all groups, butt joint tooth preparations were created in canines and molars.
Alginate-based preparations were created in another way since this polymer exhibits a basic pH and antibiotics typically precipitate in this solution.
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The 3D contour dimension of a mandibular first molar acrylic model tooth was scanned with Optical scanner (S600, Zirkonzahn, Italy), and then a classic crown preparation was created in CAD software (Zirkonzahn.Modellier, Zirkonzahn, Italy).
During the sample preparation, pools were created from equal volumes of aliquots of all samples.
During the sample preparation, pools were created from equal volumes of subaliquots of all samples in the study.
After this data preparation step/upgrade, integrated maps were created using the same JoinMap settings as used for the separate parental maps.
To assess PCR efficiency, standard curves were created by serial dilution of cDNA preparations.
Exactly for these works the data preparation system was created.
Therefore, for a proper clinical use, it is of primary importance to know what type of preparation will be created by a specific commercial system, to allow for the correct choice of a suitable final formulation.
At the time of preparation, a pool was created from equal amounts of small aliquots of each study sample, which was treated identically to a sample.
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