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These preparations were conducted in secrecy.
For a fungal culture, FISH preparations were conducted for more than ten times, from each of which eight microscope slides were resulted.
The preparations were conducted in the morning, so that the experimental bees could be released in the early afternoon.
All preparations were conducted in duplicate.
Induction of recombinant RS5 expression and crude extract preparations were conducted as previously described [ 22].
The statistical analyses for the TP-immunostained preparations were conducted as follows.
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The most robust method is likely to be one in which two independent library preparations are conducted and sequenced for each progeny individual in separate lanes.
Mixing and specimen preparation were conducted at room temperature and 50 ± 5 % relative humidity.
However, data collection, data management, statistical analyses, interpretation and manuscript preparation were conducted solely by the academic investigators without any support from pharmaceutical companies.
The analysis and the necessary data preparation were conducted using R, a language and environment for statistical computing, version 3.3.2 (R Core Team 2016).
Sample purification and NMR sample preparation were conducted in a room kept at about 20°C.
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