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Average, standard deviation, and variance for the six preparations were calculated for each parameter.
All glucocorticoid preparations were calculated to prednisone equivalent doses as described elsewhere [ 14].
The purity of the pan- and CD16+ monocyte preparations were calculated as 92.7% (IQR: 84 94.2) and 95.02% (IQR: 84.2-95.4), respectively.
Concentrations of the final preparations were calculated from an A260 reading (Beckman DU-7 spectrophotometer; Beckman Coulter Inc., Fullerton, CA, USA) and an aliquot analyzed by gel electrophoresis to ensure integrity.
Difference spectra between the oxidized and reduced mitochondrial preparations were calculated, and cytochrome a content was determined from the peak at 605 nm using an extinction coefficient of 12 l/(mol·cm) (Balaban et al., 1996).
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The porosity of pSi microparticle preparations was calculated by gravimetric analysis [42], and the microparticle thickness and pore sizes were calculated via SEM.
The active site concentration of the various RT preparations was calculated as described below.
The relative concentration of the various cDNA preparations was calculated with the 2−ΔCt method; the Q-RT PCRs were run with 1 µl of cDNA or 10-fold dilutions with the above conditions.
CoA concentration in various resin preparations is calculated to be 3 to 7 mM.
The amount of apoAI in the preparations was calculated from the [C]apoAI-specific activity.
A semiquantitative histological score for each of the slides was performed and the mean score of the five preparations was calculated.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com