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After hardening, phage preparations were applied in 10 µL aliquots directly on the soft agar with the R36A indicator strain.
Standard chromosome preparations were applied to peripheral lymphocyte cultures [57] of male individuals of human (Homo sapiens, HSA), chimpanzee (Pan troglodytes; PTR), bonobo (Pan paniscus, PPA), gorilla (Gorilla gorilla, GGO), Bornean and Sumatran orang-utan (Pongo pygmaeus, PPY), white-cheeked crested gibbon (Nomascus leucogenys; NLE), and rhesus macaque (Macaca mulatta; MMU).
Viral preparations were applied at 1 DIV.
Viral preparations were applied at 1 day in vitro (DIV).
All preparations were applied according to the manufacturer's instructions.
Predominantly dietary supplements and herbal preparations were applied, mostly without a physician's prescription.
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1 μL of these preparations was applied to the MALDI plate, and allowed to dry at rt.
For the semi-critical articles high level disinfection, formaldehyde in alcoholic at 8.0% v/v or aqueous at 10.0% v/v preparations are applied to an 18-hour exposure.
Different approaches for the layer preparation were applied.
For this purpose, planar and cross-section preparation were applied including mechanical grinding, polishing and argon ion-beam etching followed by deposition of a thin carbon film on both sides.
Briefly, 8 10 µL of protein preparation were applied to glow-discharged, carbon-coated Formvar grids (Electron Microscopy Sciences, Washington, PA) for 10 20 min. The solution was gently wicked off using Whatman grade-1 qualitative filter paper.
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