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The isolated plasmid preparations were analysed by agarose gel electrophoresis for visual inspection.
The protein preparations were analysed for homogeneity by SDS/PAGE.
The preparations were analysed with an inverted fluorescence microscope (Nikon Eclipse Ti, Melville, NY, USA).
Mitochondrial preparations were analysed for contamination with ER by Western blotting for ER-marker GRP 78.
All reactions were run in triplicate and 4 independent RNA preparations were analysed for each sample.
Chromosome preparations were analysed with the Provis AX70 Olympus microscope equipped with standard fluorescence filter sets.
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Endotoxin levels in purified protein preparations was analysed using the E-Toxate kit (Sigma).
The integrity and quality of the RNA preparations was analysed using the microcapillary fluid device form Agilent (Agilent 2100 Bioanalyzer).
Statistical differences between monocyte/macrophages from the same preparation were analysed by Student's paired t-test.
To evaluate the sensitivity and harmonisation of results between different laboratories, aliquots of a single DNA preparation were analysed at three Scandinavian laboratories.
What Brailsford does believe in is the "aggregation of marginal gains", where every little aspect of a cyclist's performance and preparation is analysed in an attempt to save crucial milliseconds.
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