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Water used in the preparations was obtained from a Millipore water purifying system.
A maximum P/N value for all three antigen preparations was obtained with an antigen dilution of 1 2000.
A collection of 25 unique m/z peaks that were significantly associated with either of the pan-monocytes or CD16+-monocyte preparations was obtained.
An RR for all such preparations was obtained by weighting the RRs of current use (0.75 for continuous, 1.05 for cyclic) accordingly, yielding an RR of 0.95 and an ERR of −0.05 (Table 6).
Sterilization of the collagen preparations was obtained by dialysis versus chloroform-containing 0.5 M acetic acid (2 ml/l) for 2 h in the cold, followed by exhaustive dialysis versus chloroform-free 0.5 M acetic acid, as reported in Luparello et al. (7).
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The more soluble superphosphate and triple superphosphate preparations are obtained by the treatment of calcium phosphate with sulfuric and phosphoric acid, respectively.
Six Natto preparations were obtained, generated by using different B. subtilis strains.
NET, DAT and SERT expressing membrane preparations were obtained from Perkin Elmer (MA, USA).
Enzyme preparations were obtained as culture supernatants from 78 fungal isolates grown on wheat straw as carbon source.
Leukemic cells were treated with C60-RITC for 2 and 18 h, washed from excess of label and fixed preparations were obtained.
Preparations were obtained squashing immature anthers in a drop of a 2% propionic acidhaematoxylin solution and using ferric acid as mordant (Núñez 1968).
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