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Activity of the various CvTAW60C preparations was evaluated in kinetic resolution of four racemic amines (rac-1a d). rac-1a d
The purity of the resulting preparations was evaluated by SDS-PAGE on 4 15% Tris-HCl gel (Bio-Rad).
The quality of all DNA preparations was evaluated by agarose gel electrophoresis.
The purity of CD34+ cell preparations was evaluated with FACS and was ≥90%.
Antimicrobial activity of examined preparations was evaluated using the agar-well diffusion assay.
Neurotoxicity of the supernatants from these preparations was evaluated in primary cultures of neurons by neutral red incorporation.
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The preparations were evaluated by means of a bright-field microscope and photographed (Optiphot 2; Nikon, Tokyo, Japan).
The technological parameters of all the preparations were evaluated using centrifugation and conductivity tests, microscopic and viscometric analysis.
The appearances of these preparations were evaluated by scanning electron microscopy (SEM), and the in vitro skin permeation of selegiline across porcine skin was examined.
Both, activity and enantioselectivity of all the immobilized lipase preparations were evaluated with different racemic esters under different reaction conditions (temperature, pH and solvent polarity).
Concentration, purity and integrity of preparations were evaluated spectrophotometrically, followed by agarose gel-ethidium bromide electrophoresis.
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CEO of Professional Science Editing for Scientists @ prosciediting.com