A full description of the development and validation process is described in another manuscript, currently in preparation (unpublished data).
The localization of the Cas1p-like protein, RWA1 (At5G46340), could not be predicted based on AmiGO data, but the RWA2 protein (At3g06550) has been found in Golgi preparations (H. Parsons and J. Heazlewood, unpublished data).
Likewise, this protein was also detected in cellulase preparations from T. reesei (unpublished data), but its function in cellulose degradation has not yet been reported.
There were no significant differences in the results of any of the experiments performed based upon the spore preparation medium (author's unpublished data).
However, preliminary analyses indicate that Parkin KO rats have a normal phenotype up to 12 months of age (our unpublished data, in preparation).
Therefore, MJFF has employed ZFN technology to knock out DJ-1 in the rat and generate an animal model that exhibits gait and locomotor deficits (our unpublished data, in preparation).
Although PINK1 knockout mouse models have been generated (Gispert et al., 2009), the PINK1 ZFN knockout rat, which displays a PD-like phenotype (our unpublished data, in preparation), can be used to test PINK1 therapeutics in a different species.
The difficulties parents had in monitoring the outdoor play and snacking behaviour of their child emerged from qualitative focus group interviews with three groups of Dutch and three groups of Turkish parents that were held during the preparation stage of the survey (unpublished data).
These results are similar to those previously observed for stimulation with crude LPS preparations in trout macrophages [[ 56], Boltaña et al., unpublished data] where both preparations can stimulate the release of TNF-α into the culture medium [[ 68], Roher et al., unpublished data].
As SHOX expression is comparatively higher in human fibroblasts (Figure 2), we further analysed an RNA-Seq dataset of Hs27 human fibroblast cells (Irena Vlatkovic and Denise Barlow, unpublished data; manuscript in preparation).
