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Despite attempts to generate pure length-defined heparin oligosaccharides, chromatographic preparations represent a heterogeneously sulfated population of oligosaccharides, thus obscuring the critical structural features of HS/heparin required to inhibit different angiogenic cytokines.
Once expression and purification was established we checked whether the large scale preparations represent also catalytically active enzyme.
Native tissue preparations represent a model in which individual cells are in histo-anatomical conditions close to the in situ setting, but where targeted intervention and observation at the cell-level are more difficult.
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In a model of T1D, expression levels were evaluated in human islet preparations, representing the target organ, as well as in human lymphocytes representing the effector cells in T1D.
Measurements of cilia length in DiffQuik-stained cells in cytospin preparations represented an assessment of the average length of the tuft of cilia at the apical end of each epithelial cell.
This method for generating hantavirus stocks using Vero E6 cells is typical and the IFNλ measured in these virus preparations represents the absolute amount of cytokine contained within a milliliter of a given virus stock.
Note, that although we don't know where and how these disulfide bonds are generated, we have at this point successfully generated tetramers from HLA preparations representing 14 different HLA alleles, and in no case have we failed.
In the above studies, the brain samples interrogated consisted of "bulk" brain tissue preparations representing cellularly heterogeneous mixes of neuronal and non-neuronal cell types.
In 2002, AHL's PRRSV ELISA was modified to include two recombinant protein preparations representing United States and European strains, but equivalent test performance and cut-off were maintained.
Hybridization probes were made from 18 total RNA preparations representing two biological replicates of the leaves of F1 21/66, F1 22/73 and Teroldego inoculated with P. viticola and collected at 12 and 96 hpi, or sprayed with water (C).
Then his kitchens cook the food New Yorkers like in an authentic Chinese preparation, representing the Sichuan, Beijing, Shanghai and Canton regions.
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